Poster Tu1747: Spatial transcriptomics reveals cellular niches associated with histological inflammation in postoperative Crohn’s disease

Robert Battat, Bruno Sangiorgi, Bryan Linggi, Sean Gui, Dax Torti, Michelle I. Smith, Saurabh Mehandru, Randy Longman, Dana J. Lukin, Ellen J. Scherl, Lihui Qin, Christopher Ma, Wendy Teft, and Niels Vande Casteele

Crohn’s disease (CD) is a chronic and debilitating condition of the gastrointestinal (GI) tract characterized by patchy, segmental, and transmural inflammation that can cause progressive GI damage and disability. Patients undergoing ileocolonic resection (ICR) often confront recurrent inflammation postoperatively, posing significant clinical hurdles. Understanding the cellular and molecular underpinnings of this recurrence remains elusive. This study aims to identify cells, locations, and transcriptional signatures associated with recurrent inflammation in CD to facilitate design of more effective therapies for patients with post-operative CD. Through spatial transcriptomic analyses (using 10X Genomics Visium CytAssist Spatial Gene Expression) of formalin-fixed paraffin-embedded (FFPE) tissue biopsies from 15 post-operative CD patients, our study revealed spatial transcriptomic signatures associated with regions of intestinal inflammation, providing an unprecedented characterization of the molecular basis of post-operative CD and potentially revealing biomarkers for disease activity and targets for therapeutic intervention.

Download this Poster to Discover:

• Uncover the cellular and molecular mechanisms driving recurrent Crohn’s Disease after ileocolonic resection (ICR).
• Explore spatial transcriptomic analyses revealing transcriptional signatures indicative of key signaling pathways across inflamed regions.
• Gain insights into the first study aligning transcriptomic signatures and cellular identities with regions of histological inflammation in patients with postoperative recurrence of Crohn’s Disease.

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