Publications
Evaluation of the effect of storage condition on cell extraction and flow cytometric analysis from intestinal biopsies
Citation
Wildenberg ME, Duijvestein M, Westera L, van Viegen T, Buskens CJ, van der Bilt JDW, Stitt L, Jairath V, Feagan BG, Vande Casteele N. Evaluation of the effect of storage condition on cell extraction and flow cytometric analysis from intestinal biopsies. J Immunol Methods. 2018 Aug;459:50-54. doi: 10.1016/j.jim.2018.05.004. Epub 2018 Jun 1. PMID: 29772249.
Abstract
Background: Flow cytometric (FC) analysis of intestinal tissue biopsies requires prompt cell isolation and processing to prevent cell death and generate valid data. We examined the effect of storage conditions prior to cell isolation and FC on viable cell yield and the proportions of immune cell phenotypes from intestinal biopsies.