Background: Flow cytometric (FC) analysis of intestinal tissue biopsies requires prompt cell isolation and processing to prevent cell death and generate valid data. We examined the effect of storage conditions prior to cell isolation and FC on viable cell yield and the proportions of immune cell phenotypes from intestinal biopsies.


Biomarker, Biopsy, Central analysis, Crohn's Disease, Cytology, Diagnosis, Dimethyl Sulfoxide, Flow cytometry, IBD, Inflammatory Bowel Disease, Intestinal Mucosa, Intestines, Multicenter clinical trial, Pathology, Translational research

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